evaluating the effects of galbanic acid on hydrogen peroxide-induced oxidative dna damage in human lymphocytes

objective: ferula szowitsiana has been widely used for medicinal purposes around the world. the anti-oxidant effect of f.szowitsiana had been proved. the current study aims to determine the protective effects of galbanic acid, a sesquiterpene coumarin from f. szowitsiana, against hydrogen peroxide (h2o2) - induced oxidative dna damage in human lymphocytes. materials and methods: human lymphocytes were incubated with h2o2 (0, 25, 50, 100, and 200 µm), galbanic acid (200 and 400 µm) and a combination of galbanic acid (200 and 400 µm) and h2o2 (25 µm) at 4 c for 30 minutes. solvents of galbanic acid without h2o2 were used as negative controls. results: the findings of this study demonstrated that h2o2 exposure leads to a significant concentration-dependent increase in dna damage. galbanic acid did not cause dna damage compared with the control cells. data showed that galbanic acid does not have a protective effect against h2o2-induced oxidative dna damage in human lymphocytes. conclusion: according to the results, it is concluded that the capability of f. szowitsiana in reducing reactive oxygen species and the anti-inflammatory property of its methanolic extract may be due to its other ingredients.